P918 Clostridium butyricum inhibits epithelial-mesenchymal transition of intestinal carcinogenesis through downregulating METTL3

Abstract Background Colorectal cancer (CRC) is related to gut microbiota dysbiosis, especially butyrate-producing bacteria reduction. Our previous study suggested administration of Clostridium butyricum (C. butyricum), a bacterium, exerts crucial effect against CRC. Methyltransferase-like 3 (METTL3) contributes tumorigenic epigenetic regulation. We aimed investigate the effects C. on METTL3 in prevention Methods TCGA and CancerSEA databases were used co-expressed genes, GO KEGG enrichment analysis was conducted METTL3. Tissue specimens human normal colonic tissue, adenoma carcinoma obtained examined expression METTL3, EMT-associated markers, VM formation. overexpressed CRC cells assess cell migration tube Meanwhile, epithelial-mesenchymal transition (EMT), vasculogenic mimicry (VM) formation evaluated. Furthermore, murine xenograft model established provide further evidence inhibition tumor growth. Results Database that showed positive correlation with proliferation, EMT, DNA repair, metastasis, invasion. The gradually increased from colon carcinoma, positively correlated EMT. overexpression promoted migration, invasion induced could downregulate decrease vimentin vascular endothelial growth factor receptor 2 reduce EMT Moreover, alleviated pro-oncogenic overexpressing plasmid cells. Accordingly, downregulated tumors prevented nude mice. Conclusion inhibit intestinal carcinogenesis through downregulating These findings broaden our understanding probiotics supplement treatment